Mukherjee, who was a popular face on the Bengali silver screen from the 1970s to 1990, died at a private hospital in Mumbai.
Away from films, Mukherjee had reportedly moved to Mumbai permanently after selling her residence in Kolkata.
From Shesh Parba to Marjina Abdulla
Mukherjee made her Bengali film debut with Chitta Bose's Shesh Parba in the early 1970s.
Her breakthrough came with Dinen Gupta's Marjina Abdulla, in which she played the titular character opposite Debraj Roy. The film established her as a popular actress and was followed by a series of successful Bengali films.
One of her most remembered films was Arabinda Mukhopadhyay's Mouchak (1974), in which she starred opposite Ranjit Mallick.
Mithu Mukherjee sharing screen-space with Ranjit Mallick in Mouchak. Photo: Screen-grab
The film became a major commercial success and remains one of the titles most closely associated with Mukherjee's Bengali film career.
She also shared the screen with actors including Soumitra Chattopadhyay, Debraj Roy and Subhendu Chattopadhyay during her career.
Foray into Hindi cinema
Mukherjee also worked in Hindi cinema. Her Hindi debut was Dulal Guha's Khaan Dost (1976).
She subsequently appeared in films associated with director Basu Chatterjee, including Safed Jhoot, Dillagi and Do Ladke Dono Kadke.
In Dillagi, she appeared opposite Shatrughan Sinha, while Do Ladke Dono Kadke featured her opposite Navin Nischol.
Mithu Mukherjee in Dillagi. Photo: Screen-grab
A career beyond glamour
Beyond her glamorous and mainstream roles, Mukherjee received critical appreciation for her performance in the Bengali film Father (1981).
After Dujane, Mukherjee took a lengthy break from acting. She eventually returned with Chandra Barot's Ashrita (1990), a woman-centric social drama that became a commercial success.
Ashrita became her final film. After its release, Mukherjee chose to leave the film industry and live away from the public spotlight.
Unlike many of her contemporaries, she largely stayed away from public life after ending her acting career.

Charming screen presence with teasing sparkle
Mithu Mukherjee had a screen presence that was difficult to imitate — vibrant, spirited and mischievous, with a teasing sparkle that made her instantly appealing.
There was an unmistakable sensuality to her performances, but it was rarely forced. She carried her femininity with confidence, combining flirtatious charm with warmth and an innate sense of decency.
Her smile could be playful, her expressions impish, and there was often a sense that she knew exactly the effect she was creating on screen.
That combination of sensuality, innocence, wit and mischief gave her a personality that stood apart from the conventional heroines of her era.
She was not merely glamorous; she was lively, unpredictable and distinctly her own — qualities that helped make her memorable among the leading actresses of Bengali cinema of her generation.
Mithu Mukherjee in Swayamsiddha. Photo: Screen-grab
Prosenjit Chatterjee mourns Mithu Mukherjee
Mourning her demise, Bengali film superstar Prosenjit Chatterjee wrote on X, “The news of Mithu Mukhopadhyay's passing is extremely heartbreaking. His memory and his work will live on forever among us. With deepest respects.”
মিঠু মুখোপাধ্যায়ের প্রয়াণের খবর অত্যন্ত বেদনাদায়ক। তাঁর স্মৃতি ও তাঁর কাজ আমাদের মধ্যে চিরকাল বেঁচে থাকবে। গভীর শ্রদ্ধা। pic.twitter.com/IUNbvHnXC1
— Prosenjit Chatterjee (@prosenjitbumba) September 21, 2026